STAT3-Luc/HEK293
CBPB0002
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| I. Background | |
| 癌癥相關炎癥的特征是存在特定的炎癥細胞和炎癥介質(zhì)，包括細胞因子和趨化因子。 最近的證據(jù)表明，信號轉(zhuǎn)導和轉(zhuǎn)錄激活因子（STAT）家族蛋白，特別是 STAT3，在惡性轉(zhuǎn) 化開始和癌癥進展過程中，在選擇性誘導和維持癌前炎癥微環(huán)境中起著至關重要的作用。 信號轉(zhuǎn)導和轉(zhuǎn)錄激活因子 3 (STAT3)是一種轉(zhuǎn)錄激活因子，它將細胞外受體的信號直 接傳遞到細胞核，介導一些基因的表達，在細胞凋亡等細胞過程中發(fā)揮重要作用。在細胞 因子和生長因子的作用下，STAT3 被受體相關 Janus 激酶(JAK) 磷酸化，形成同二聚體或異 二聚體，并轉(zhuǎn)位到細胞核中，作為轉(zhuǎn)錄激活劑發(fā)揮作用。STAT3 信號傳導是惡性腫瘤的主 要內(nèi)在途徑，因為它在惡性細胞中頻繁激活，并且在調(diào)節(jié)腫瘤微環(huán)境中對癌癥發(fā)炎至關重 要的許多基因中起關鍵作用。 |
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| II. Description | |
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STAT3-Luc HEK293 報告基因細胞，是由 STAT3 調(diào)控并表達 Luc 熒光素 酶報告基因的HEK293細胞。IL6細胞因子與受體復合物結(jié)合，啟動JAK/STAT3信號通路， 原理見下圖所示。
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| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | STAT3-Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Acute-Phase Response Factor, DNA-Binding Protein APRF, DNA, ADMIO, Signal Transducer And Activator Of Transcription 3, TATA |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM + 10%FBS+100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid |
| Growth Properties: | Adherent |
Ⅴ. Representative Data |
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Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Induction of STAT3 activity by hIL6 in STAT3-Luc HEK293 Cells (C7).  |
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Figure 3. Inhibition of IL-6-induced Reporter Activity by IL-6 Neutralizing Antibody in STAT3-Luc HEK293 Cells. |
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