CRE-Luc/HEK293
CBP74027
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I. Background | |
cAMP 反應(yīng)元件結(jié)合蛋白 (CREB) 位于細(xì)胞核內(nèi),是一種轉(zhuǎn)錄因子,在 Ser133 位點(diǎn)被不同的受體活化蛋白激酶(如蛋白激酶 A (PKA)、鈣調(diào)蛋白依賴性蛋白激酶 (CaMK)、絲裂原活化蛋白激酶 (MAPK) 和其他激酶磷酸化后,與靶基因啟動(dòng)子的 cAMP 反應(yīng)元件 (CRE)結(jié)合。CRE 是 CREB 的主要結(jié)合位點(diǎn),負(fù)責(zé)激活 CREB。CRE 是許多細(xì)胞外和細(xì)胞內(nèi)信號(hào)通路的焦點(diǎn),包括 cAMP、鈣、GPCR(G 蛋白偶聯(lián)受體)和神經(jīng)營養(yǎng)因子。cAMP/PKA 信號(hào)通路對(duì)許多生命過程和生物體至關(guān)重要。在 cAMP/PKA 信號(hào)通路中,CREB 通過 PKA 的磷酸化被激活,并與 CRE 結(jié)合,一般基序?yàn)?5'-TGACGTCA-3'。由于 CRE 是 cAMP/PKA信號(hào)通路的調(diào)節(jié)劑,因此可以研究各種抑制劑的作用。 | |
II. Description | |
CRE-Luc HEK293 報(bào)告基因細(xì)胞是由 CRE 調(diào)控并表達(dá) Luc 熒光素酶報(bào)告基因的 HEK293 細(xì)胞。Forskolin 是天然存在的直接腺苷酸環(huán)化酶(AC)/cAMP/CREB 的 激活劑,原理見下圖所示。 Figure 1:CRE-Luc HEK293 原理示意圖 |
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III. Introduction | |
Host Cell: | HEK293 |
Expressed gene: | CRE-Luciferase |
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Synonym(s): | CRE cell line, CREB cell line, luciferase reporter |
Freeze Medium: | 90% FBS+10% DMSO |
Culture Medium: | DMEM+10%FBS+150ug/ml hygromycin |
Mycoplasma Testing: | Negative |
Storage: | Liquid nitrogen |
Application(s): | • Monitor cAMP/PKA signaling pathway activity. • Screen for activators or inhibitors of the cAMP/PKA signaling pathway. |
IV. Description of Host Cell Line | |
Organism: | Homo sapiens, human |
Tissue: | Embryonic kidney |
Disease: | Normal |
Morphology: | Epitheloid cell |
Growth Properties: | Adherent |
Ⅴ. Representative Data | |
Figure 2. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Induction of CRE activity by Forskolin in CRE-Luc HEK293(C5). |