NFκB-Luc/HEK293
CBP74026
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| I. Background | |
| 活化 B 細(xì)胞的核因子 κ 輕鏈增強(qiáng)子( NF-κB ) 是一個(gè)轉(zhuǎn)錄因子蛋白復(fù)合物家族，可控制 DNA 轉(zhuǎn)錄、細(xì)胞因子產(chǎn)生和細(xì)胞存活。NF-κB 存在于幾乎所有動(dòng)物細(xì)胞類(lèi)型中，并參與細(xì) 胞對(duì)壓力、細(xì)胞因子、自由基、重金屬、紫外線照射、氧化 LDL 以及細(xì)菌或病毒抗原等刺激的反應(yīng)。 NF-κB 的激活涉及兩條主要信號(hào)通路，即經(jīng)典通路和非經(jīng)典（或替代）通路，盡管它 們的信號(hào)機(jī)制不同，但都對(duì)調(diào)節(jié)免疫和炎癥反應(yīng)十分重要。經(jīng)典 NF-κB 激活的主要機(jī)制是通過(guò)多亞基 IκB 激酶 (IKK) 復(fù)合物對(duì) IκBα 的位點(diǎn)特異性磷酸化引發(fā)的誘導(dǎo)性降解。與經(jīng)典 NF-κB 通路相反，非經(jīng)典 NF-κB 通路選擇性地對(duì)一組特定的刺激作出反應(yīng)，包括 TNFR 超 家族成員子集的配體，例如 LTβR、BAFFR、CD40 和 RANK。 |
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| II. Description | |
NFκB-Luc HEK293 報(bào)告基因細(xì)胞，是由 NFκB 調(diào)控并表達(dá) Luc 熒光素酶 報(bào)告基因的 HEK293細(xì)胞。PMA是 DAG（二酯酰甘油）的類(lèi)似物，可自由的通過(guò)細(xì)胞膜， 直接作用于細(xì)胞活化信號(hào)轉(zhuǎn)導(dǎo)通路上的 PKC，原理見(jiàn)下圖所示。 Figure 1：NFκB-Luc HEK293 細(xì)胞模型原理圖 |
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| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | NF-κB-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +100ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
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IV. Description of Host Cell Line |
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| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 2.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Induction of NF-κB activity by PMA with Ionomycin in NFκB-Luc HEK 293 Cell. |
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