I. General information |
Synonyms: |
KYSE-150 |
Background: |
established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice |
Species: |
human (Homo sapiens) |
Tissue: |
esophageal |
Disease: |
esophageal squamous cell carcinoma |
Gender: |
N/A |
Morphology: |
epitheloid cells growing as adherent monolayer; |
Growth Mode: |
N/A |
Doubling Time: |
below 25 hours (even less when serum amount is increased) |
DNA Profile: |
N/A
Cobioer’s Cell Line Authentication Service |
Culture Medium: |
RPMI 1640+10%FBS
KYSE-150完全培養(yǎng)基,# CBP60733M
We strongly suggest to purchase the complete medium from Cobioer.
|
Cryopreservation medium: |
90%FBS+10%DMSO |
Mycoplasma: |
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: |
cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - |
Fingerprint: |
multiplex PCR of minisatellite markers revealed a unique DNA profile |
Species: |
confirmed as human with IEF of AST, LDH, MDH |
Cytogenetics: |
human hypertriploid karyotype with 12% polyploidy - 70(68-75)<3n>XderX, -X, +1, -3, -4, -8, -9, +11, -14, -15, -16, -18, -21, +12mar (4xbisat), add(X)(q27), inv(1)(p34q32), del(1)(p32), del(1)(q11), del(7)(q32), del(8)(p21), i(9q), add(9)(q34), add(11)(p11), der(11)add(11)(p1?)add(11)(q24), del(1)(p11), del(12)(q21), del(15)(q23q25), add(19)(q13) |
Virus |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
Comments: |
For more information, please contact Cobioer (4008-750-250). |