TLR3/NFκB-Luc/HEK293
CBP74057
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| I. Background | |
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TLR3 (Toll Like Receptor 3) is a Protein Coding gene. Diseases associated with TLR3 include Encephalopathy, Acute, Infection-Induced 2 and Human Immunodeficiency Virus Type 1. Among its related pathways are Activated TLR4 signalling and Toll-like Receptor Signaling Pathway. Gene Ontology (GO) annotations related to this gene include identical protein binding and transmembrane signaling receptor activity. |
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| II. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | TLR3、NFκB-Luc |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: |
MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
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| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). TLR3/NF-κB Reporter-HEK293 cells were stimulated by Poly(I:C), the S/B was 57-fold.
